J Hematol

Journal of Hematology, ISSN 1927-1212 print, 1927-1220 online, Open Access

Article copyright, the authors; Journal compilation copyright, J Hematol and Elmer Press Inc

Journal website http://www.thejh.org

Original Article

Volume 4, Number 1, March 2015, pages 125-130

Attached Segments and Cryovial Samples as a Useful Tool in Cord Blood Banking Quality Control

Figures

Figure 1. Linear correlation between CD34⁺/UCB unit and CD34⁺/QC cryovial.

Figure 2. Linear correlation between CD34⁺/UCB unit and CD34⁺/attached segment.

Tables

**Table 1.** UCB Content and TNC Percent of Viability Before Cryopreservation and After Thawing and Its Correlation With Corresponding Values of Attached Segment and QC Sample
Variable	Fresh		After thawing		Attached segment				QC sample
Variable	Mean ± SD	Range	Mean ± SD	Range	Mean ± SD	Range	P	R²	Mean ± SD	Range	P	R²
Associated probability for the two-tailed Student’s t-test and Pearson correlation coefficient have been calculated. *ECLONE is defined as the CFU/CD34⁺ ratio of a given UCB/segment/QC sample.
TNC (× 10⁶)	768.40 ± 90.57	613 - 926	636.4 ± 94.42	483 - 808	595.57 ± 80.54	451 - 710	0.08	0.92	597.5 ± 91.41	450 - 783	0.11	0.98
CD34⁺ (× 10⁶)	1.02 ± 0.33	0.41 - 1.53	0.93 ± 0.33	0.35 - 1.45	0.89 ± 0.29	0.40 - 1.46	0.62	0.98	0.90 ± 0.33	0.33 - 1.43	0.75	0.99
CFU (× 10⁴)	92.84 ± 26.02	46.23 - 135.69	82.69 ± 24	36.49 - 128.13	74.52 ± 18.08	37.68 - 101.32	0.14	0.93	75.47 ± 19.81	37.10 - 106.57	0.21	0.97
TNC % viability	94.93 ± 1.96	92 - 98	90.57 ± 1.98	87 - 94	89.4 ± 3.15	84.40 - 96.6	0.09	0.80	89.43 ± 2.7	84.10 - 95.20	0.07	0.89
ECLONE*	0.95 ± 0.23	0.57 - 1.55	0.84 ± 0.20	0.53 - 1.48	0.82 ± 0.26	0.51 - 1.45	0.65	0.81	0.83 ± 0.20	0.52 - 1.46	0.19	0.93

**Table 2.** Percentage of CFU and Cell (TNC and CD34⁺) Recovery After Thawing for UCB, Attached Segment and QC Sample
Variable	UCB		Attached segment			QC sample
Variable	Mean ± SD	Range	Mean ± SD	Range	R²	Mean ± SD	Range	R²
TNC % recovery	82.62 ± 4.42	69.1 - 89.1	81.09 ± 5.36	67.66 - 88.68	0.89	81.97 ± 5.49	68.41 - 89.06	0.90
CD34⁺ % recovery	89.75 ± 5.27	76.1 - 97.7	88.39 ± 7.57	74.2 - 96.8	0.89	89.27 ± 9.92	75.5 - 97.3	0.92
CFU total % recovery	88.76 ± 4.2	78.9 - 100	86.16 ± 9.38	75.4 - 100	0.87	87.34 ± 7.3	76.1 - 100	0.88