A Comparative Study on Platelet Activation Markers Between Continuous and Intermittent Exercise Training Programs in Healthy Males

Abstract

Background: Platelet plays an important role in the hemostatic system. The hyper-activation of platelet may lead to the development of thrombotic event in cardiovascular disease (CVD). Aerobic exercise is well known to reduce CVD risk and can reduce the platelet activation. The purpose of this study was to investigate the effect of the continuous and intermittent training programs on platelet activation.

Methods: There were 24 healthy sedentary males mean aged 21.75 3.73 years old participating in this study. They were divided into three groups, which are control group (n = 9), continuous group (n = 7) and intermittent group (n = 8). Continuous training group cycled at 60% VO₂ max for 45 minutes. For the intermittent training group, participants cycled at the 45% VO₂ max and had a rest before the second session at 75% VO₂ max, and the sessions were repeated twice. The exercise was performed non-stop until 45 minutes. Both exercise training groups performed the exercise three times per week for 12 weeks. Platelet activation was measured via detection of the P-selectin and glycoprotein IIb-IIIa using flow cytometry by indirect immunoflourescence.

Results: This study showed that, intermittent type of training showed a significant difference of the gated percentage of CD62p and PAC-1 activation. There was also a significant difference of PAC-1 after training between two groups.

Conclusions: As a conclusion, intermittent group pronounced more beneficial effects on platelet activation, whereas continuous group provided the beneficial changes on platelet activation.

J Hematol. 2014;3(3):72-75
doi: http://dx.doi.org/10.14740/jh163w